2LYN
HIGH RESOLUTION STRUCTURE OF RED ABALONE LYSIN DIMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 72.520, 72.520, 266.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 * - 2.070 |
| R-factor | 0.213 |
| R-free | 0.28500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lyn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.550 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 2.180 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.087 * | 0.256 * |
| Total number of observations | 948262 * | |
| Number of reflections | 44137 | |
| <I/σ(I)> | 4.8 | 2.8 |
| Completeness [%] | 99.1 | 94.6 |
| Redundancy | 6.9 * | 4.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 * | 295 * | 0.75 M (NH4)2SO4, 20 MM NH4SCN, 52.5 MM SODIUM-CITRATE-BORIC ACID BUFFER PH 4.5, 0.1 MM EDTA, 0.02% OTGP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | 0.1 (%(w/v)) | ||
| 4 | 1 | reservoir | ammonium sulfate | 0.75 (M) | |
| 5 | 1 | reservoir | 20 (mM) | ||
| 6 | 1 | reservoir | sodium citrate/boric acid/citric acid | 52.5 (mM) | |
| 7 | 1 | reservoir | EDTA | 0.1 (mM) | |
| 8 | 1 | reservoir | 1-S-octyl-beta-D-thioglucopyranoside | 0.02 (%) |
