2LVE
RECOMBINANT LEN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01 |
| Detector | FUJI |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.400, 104.400, 58.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lve |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.820 |
| High resolution limit [Å] | 2.700 | 2.690 |
| Rmerge | 0.061 | 0.346 |
| Number of reflections | 5571 | |
| <I/σ(I)> | 26 | 9.2 |
| Completeness [%] | 96.6 | 97.2 |
| Redundancy | 1.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 30 (%) | |
| 3 | 1 | drop | sodium citrate | 0.1 (M) | |
| 4 | 1 | drop | sodium acetate | 0.2 (M) | |
| 5 | 1 | drop | zinc sulfate | 0.02 (M) |
