2LTN
DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.730, 61.160, 136.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.700 |
| R-factor | 0.177 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.037 |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | PEG3350 | 2 (%(w/v)) | |
| 3 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 4 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 40 (%(w/v)) |
