2LIV
PERIPLASMIC BINDING PROTEIN STRUCTURE AND FUNCTION. REFINED X-RAY STRUCTURES OF THE LEUCINE/ISOLEUCINE/VALINE-BINDING PROTEIN AND ITS COMPLEX WITH LEUCINE
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.880, 70.990, 115.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.179 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.046 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 * |
| Rmerge | 0.021 * |
| Total number of observations | 41179 * |
| Number of reflections | 12004 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | drop | sodium citrate | 10 (mM) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | |
| 6 | 1 | drop | PEG6000 | 20 (%) | |
| 7 | 1 | reservoir | PEG6000 | 18 (%) | |
| 8 | 1 | reservoir | 0.02 (%) | ||
| 9 | 1 | reservoir | sodium citrate | 10 (mM) |
