2LIS
HIGH RESOLUTION STRUCTURE OF THE RED ABALONE LYSIN MONOMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.410, 45.310, 81.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.350 |
| R-factor | 0.1358 |
| R-free | 0.17870 |
| Structure solution method | OTHER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.021 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 1.380 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.045 * | 0.082 * |
| Total number of observations | 259591 * | |
| Number of reflections | 41576 | |
| <I/σ(I)> | 7.2 | 8.6 |
| Completeness [%] | 97.4 | 88.7 |
| Redundancy | 4.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 * | 295 * | 1.26 M AMMONIUM SULFATE, 100 MM CHESS PH 9.5, 200 MM NACL, pH 9.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | 0.1 (%(w/v)) | ||
| 4 | 1 | reservoir | ammonium sulfate | 0.75 (M) | |
| 5 | 1 | reservoir | 20 (mM) | ||
| 6 | 1 | reservoir | sodium citrate/boric acid/citric acid | 52.5 (mM) | |
| 7 | 1 | reservoir | EDTA | 0.1 (mM) | |
| 8 | 1 | reservoir | 1-S-octyl-beta-D-thioglucopyranoside | 0.02 (%) |
