2LBD
LIGAND-BINDING DOMAIN OF THE HUMAN RETINOIC ACID RECEPTOR GAMMA BOUND TO ALL-TRANS RETINOIC ACID
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 268 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.600, 60.600, 155.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.060 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.31300 |
| Structure solution method | SIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 21.600 * |
| Data reduction software | MARXDS |
| Data scaling software | MARSCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.990 | 2.100 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.097 | 0.332 |
| Number of reflections | 17193 | |
| <I/σ(I)> | 22.8 | 3.64 |
| Completeness [%] | 94.1 | 57.5 |
| Redundancy | 3.77 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 15 * | VAPOR DIFFUSION METHOD, HANGING DROP TECHNIQUE 5UL PROTEIN SOLUTION + 5UL RESERVOIR AGAINST 500UL RESERVOIR, pH 7.0, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 0.75 (M) | |
| 10 | 1 | reservoir | PIPES | 100 (mM) | |
| 2 | 1 | drop | PIPES | 50 (mM) | |
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 5 (mM) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | CHAPS | 1 (mM) | |
| 7 | 1 | drop | n-dodecyl-beta-D-maltoside | 0.08 (mM) | |
| 8 | 1 | drop | glycerol | 2 (%) | |
| 9 | 1 | reservoir | sodium acetate | 1.5 (M) |
