2KZZ
KLENOW FRAGMENT WITH NORMAL SUBSTRATE AND ZINC ONLY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 101.700, 101.700, 85.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25000 * |
| Structure solution method | RIGID-BODY REFINEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.068 * | 0.224 * |
| Total number of observations | 173188 * | |
| Number of reflections | 29618 * | |
| <I/σ(I)> | 12.8 | 2.2 |
| Completeness [%] | 84.6 | 90.1 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 18 * | Brick, P., (1983) J. Mol. Biol., 166, 453. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CITRATE | ||
| 2 | 1 | 2 | SODIUM CITRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (%) | |
| 2 | 1 | drop | sodium citrate | 1.1-1.2 (M) |
