2KAU
THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 170.800, 170.800, 170.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.22500 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.980 |
| Data reduction software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.089 |
| Total number of observations | 386731 * |
| Number of reflections | 58334 |
| Completeness [%] | 93.0 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | 1.6 (M) |
