2JLJ
Crystal Structure of the cytoplasmic domain of Yersinia pestis YscU N263A P264A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.041, 66.041, 70.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.280 - 1.300 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.340 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.080 | 0.590 |
| Number of reflections | 37461 | |
| <I/σ(I)> | 19.2 | 1.6 |
| Completeness [%] | 95.5 | 66.5 |
| Redundancy | 8.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100 MM HEPES PH 7, 1 M SODIUM MALONATE PH 7, 0.75 M SODIUM CHLORIDE |
