2JJY
Crystal structure of Francisella tularensis enoyl reductase (ftFabI) with bound NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.006, 99.454, 111.032 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.320 - 2.900 |
| R-factor | 0.206 |
| Rwork | 0.202 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.551 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019 24/04/2001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.320 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.180 | 0.580 |
| Number of reflections | 24222 | |
| <I/σ(I)> | 10.6 | 3.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PEG 3350, MG ACETATE., pH 8 |
