2JJW

Structure of human signal regulatory protein (sirp) gamma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-16
Detector	MARRESEARCH
Spacegroup name	P 64 2 2
Unit cell lengths	76.390, 76.390, 78.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.210 - 1.700
R-factor	0.23
Rwork	0.228
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2UV3 CHAIN A
RMSD bond length	0.011
RMSD bond angle	1.362
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.200	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.050	0.730
Number of reflections	14652
<I/σ(I)>	43.9	7
Completeness [%]	95.2	99.8
Redundancy	32.3	33.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		300 NL 11.5 MG/ML SIRP GAMMA PLUS 50 NL RESERVOIR (2.0 M AMMONIUM SULPHATE, 0.1 M BIS-TRIS PH 6.5) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 20.5 C.