2JJV
Structure of human signal regulatory protein (sirp) beta(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-16 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.979, 47.490, 40.044 |
| Unit cell angles | 90.00, 93.39, 90.00 |
Refinement procedure
| Resolution | 57.350 - 1.800 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2UV3 CHAIN A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.170 |
| Number of reflections | 19509 | |
| <I/σ(I)> | 30 | 4.4 |
| Completeness [%] | 96.8 | 79.1 |
| Redundancy | 5.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 300 NL 25 MG/ML SIRP BETA PLUS 100 NL RESERVOIR (3.2 M AMMONIUM SULPHATE, 0.1 M MES PH 6.0) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 20.5 C. |
