2JJR
V232K, N236D-trichosanthin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-29 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.640, 73.640, 143.443 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.760 - 2.300 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tcs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.385 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MOSFLM |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.670 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.200 |
| Number of reflections | 20607 | |
| <I/σ(I)> | 17.8 | 4.9 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2% PEG 8000, 1.0 M LITHIUM SULFATE |
