2JJQ
The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C5)- methyltransferase in complex with S-adenosyl-L-homocysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.894, 63.313, 101.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.760 - 1.800 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.713 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.480 |
Number of reflections | 33008 | |
<I/σ(I)> | 22 | 3.7 |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 5.2 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 15% PEG 8000, 0.05 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE PH 5.6 |