2JJL
Structure of avian reovirus sigmaC117-326, P321 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 77.657, 77.657, 121.449 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN C OF PDB ENTRY 2VRS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 19318 | |
| <I/σ(I)> | 52.6 | 10.4 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 20 | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.4 | 0.6-0.75 M AMMONIUM SULPHATE, 0.1 M TRIS-HCL PH 8.4, 25% GLYCEROL, 50 MM ZINC SULPHATE |
