2JJ4
The complex of PII and acetylglutamate kinase from Synechococcus elongatus PCC7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-07 |
| Detector | ADSC CCD Q4R |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.901, 149.539, 162.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.460 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2BTY AND 1QY7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.132 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.070 | 3.650 |
| High resolution limit [Å] | 3.460 | 3.460 |
| Rmerge | 0.100 | 0.390 |
| Number of reflections | 17338 | |
| <I/σ(I)> | 6.3 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M SODIUM CACODYLATE PH 6.5, 0.25M MAGNESIUM ACETATE, 10%(WT/VOL) POLYETHYLENE GLYCOL 8K, 20MM ACETYLGLUTAMATE |
