2JIN
Crystal structure of PDZ domain of Synaptojanin-2 binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 65.993, 65.993, 41.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.680 - 1.500 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fe5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.418 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.680 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 14288 | |
| <I/σ(I)> | 12.6 | 2.1 |
| Completeness [%] | 99.7 | 97.6 |
| Redundancy | 4.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 30% PEG10K, 0.20M LITHIUM SULPHATE, 0.1M ACETATE PH 4.5 |
