2JIK
Crystal structure of PDZ domain of Synaptojanin-2 binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.482, 38.335, 60.745 |
| Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.350 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fe5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.315 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.350 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 35482 | |
| <I/σ(I)> | 17.3 | 5.3 |
| Completeness [%] | 97.7 | 97.4 |
| Redundancy | 6.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 20% PEG6K, 1M LITHIUM CHLORIDE, 0.1M CITRATE PH4.0, pH 4.00 |
