2JIJ
Crystal structure of the apo form of Chlamydomonas reinhardtii prolyl- 4 hydroxylase type I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.320, 117.460, 72.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.830 - 2.900 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.705 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 69167 | |
| <I/σ(I)> | 9.7 | 1.8 |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
