2JII
Structure of vaccinia related kinase 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.258, 54.423, 91.822 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ENSEMBLE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 60969 | |
| <I/σ(I)> | 12.5 | 2.2 |
| Completeness [%] | 99.2 | 95.4 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH=7.5 2.0 M AMMONIUMFORMATE, pH 7.50 |
