2JIF
Structure of human short-branched chain acyl-CoA dehydrogenase (ACADSB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 150.245, 150.245, 201.586 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.000 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.360 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.660 |
| Number of reflections | 175824 | |
| <I/σ(I)> | 12.7 | 2.6 |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 6.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 30% PEG6000, 0.15M AMMONIUM CHLORIDE PH6.3, 10% ETHYLENE GLYCOL, pH 6.30 |
