2JIC
High resolution structure of xylanase-II from one micron beam experiment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.570, 38.830, 57.340 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 11.050 - 1.500 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d97 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.160 |
| Number of reflections | 26872 | |
| <I/σ(I)> | 14.3 | 7.1 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 2M AMMONIUM SULPHATE, 100 MM MES PH 5.5 AND 0.5 M MGCL2 |
