2JHV
CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-02 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.687, 130.889, 92.822 |
| Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
| Resolution | 92.850 - 2.070 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxw |
| RMSD bond length | 0.037 |
| RMSD bond angle | 2.643 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 87249 | |
| <I/σ(I)> | 10.3 | 2.6 |
| Completeness [%] | 94.4 | 99.8 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5 |
