2JHP

The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-05
Detector	ADSC CCD
Wavelength(s)	0.933, 0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	76.242, 76.242, 421.508
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.229
Rwork	0.226
R-free	0.28900
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.552
Data scaling software	SCALEPACK
Phasing software	SHELXCD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.150	0.540
Number of reflections	26662
<I/σ(I)>	26	1.2
Completeness [%]	99.9	99.5
Redundancy	24.4	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7		100 MM IMIDAZOLE PH 8.0, 2%-8% PROPAN-2-OL, 0-250 MM NACL, 50 MM DTT, 2 MM ADOHCY, 5MG/ML VP4 CONCENTRATION, SITTING DROP.