2JHO
Cyanomet Sperm Whale Myoglobin at 1.4A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2005-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.409, 29.786, 63.633 |
| Unit cell angles | 90.00, 104.93, 90.00 |
Refinement procedure
| Resolution | 61.430 - 1.400 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.040 | 0.180 |
| Number of reflections | 22431 | |
| <I/σ(I)> | 32 | 5 |
| Completeness [%] | 93.9 | 72 |
| Redundancy | 6.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2 M AMMONIUM SULPHATE AND 0.05 M PHOSPHATE BUFFER (PH 6.5) |
