2JHH
Structure of globular heads of M-ficolin at acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 |
| Unit cell lengths | 69.400, 69.400, 77.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.030 - 1.700 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.030 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 45425 | |
| <I/σ(I)> | 14.9 | 3.9 |
| Completeness [%] | 98.7 | 95.5 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | pH 5.6 |
