2JHE
N-terminal domain of TyrR transcription factor (residues 1 - 190)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.456, 72.009, 96.814 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 24.400 - 2.300 |
| R-factor | 0.252 |
| Rwork | 0.248 |
| R-free | 0.31700 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.871 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.400 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | |
| Number of reflections | 40199 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 98.2 | 94.6 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 0.31 M AMMONIUM SULFATE, 33% PEG-MME 5000, 0.1 M MES, PH 6.3 |
