2JHA
The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-02 |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 75.400, 75.400, 422.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.400 |
R-factor | 0.245 |
Rwork | 0.240 |
R-free | 0.33100 |
Structure solution method | OTHER |
RMSD bond length | 0.018 |
RMSD bond angle | 1.901 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.180 | 0.580 |
Number of reflections | 10116 | |
<I/σ(I)> | 13.7 | 1.4 |
Completeness [%] | 99.6 | 97.6 |
Redundancy | 8.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.7 | 100 MM IMIDAZOLE PH 8.0, 2%-8% PROPAN-2-OL, 0-250 MM NACL, 50 MM DTT |