2JH8
The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 75.055, 75.055, 419.834 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.220 |
| R-factor | 0.24553 |
| Rwork | 0.246 |
| R-free | 0.34125 |
| Structure solution method | OTHER |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.956 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.210 | 0.680 |
| Number of reflections | 11731 | |
| <I/σ(I)> | 12.4 | 1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 18.2 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 100 MM IMIDAZOLE PH 8.0, 2%-8% PROPAN-2-OL, 0-250 MM NACL, 50 MM DTT |
