2JGS
Circular permutant of avidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.768, 79.309, 71.727 |
| Unit cell angles | 90.00, 98.73, 90.00 |
Refinement procedure
| Resolution | 70.890 - 1.900 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2avi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.648 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 36425 | |
| <I/σ(I)> | 12 | 3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
