2JGQ

Kinetics and structural properties of triosephosphate isomerase from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 2
Unit cell lengths	128.167, 76.657, 49.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.730 - 2.300
R-factor	0.2
Rwork	0.200
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mo0
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.350
Number of reflections	22125
<I/σ(I)>	29.3	7.1
Completeness [%]	99.2	97.6
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		pH 8