2JGP

Structure of the TycC5-6 PCP-C bidomain of the tyrocidine synthetase TycC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-01
Detector	MAR
Spacegroup name	P 43 21 2
Unit cell lengths	84.973, 84.973, 164.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 1.850
R-factor	0.209
Rwork	0.208
R-free	0.23800
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	0.963
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.040	0.580
Number of reflections	51834
<I/σ(I)>	20.9	2.2
Completeness [%]	98.9	100
Redundancy	5.05

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		7.5 MG PROTEIN/ML MIXED 1:1 WITH A RESERVOIR SOLUTION CONTAINING 1.6 M AMMONIUM SULFATE, 0.04 M MES (PH 6.5) AND 4% DIOXANE