2JGD
E. COLI 2-oxoglutarate dehydrogenase (E1o)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-15 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 142.493, 142.493, 251.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.090 - 2.600 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.079 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.140 | 0.870 |
| Number of reflections | 80455 | |
| <I/σ(I)> | 17.2 | 3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.7 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | SITTING DROP: 1UL 12% PEG 4000, 50MM SOCIUM CITRATE PH5.6. 1UL 10MG/ML TE1O. RESERVOIR: 1UL 12% PEG 4000, 50MM SOCIUM CITRATE PH5.6., pH 5.60 |
