2JGC

Structure of the human eIF4E homologous protein, 4EHP without ligand bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-10-05
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	43.499, 49.299, 103.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.710 - 2.400
R-factor	0.212
Rwork	0.210
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jgb
RMSD bond length	0.013
RMSD bond angle	1.312
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.130	0.480
Number of reflections	34849
<I/σ(I)>	10.6	2.5
Completeness [%]	98.6	96.6
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		20% PEG-3000, 0.1M SODIUM CITRATE PH 5.5