2JFX

Crystal structure of Helicobacter pylori glutamate racemase in complex with D-Glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-12-16
Detector	MARRESEARCH
Wavelength(s)	0.9700 0.97821, 0.91834
Spacegroup name	P 21 21 21
Unit cell lengths	62.180, 81.070, 113.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.228
R-free	0.28080
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.360
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	RANTAN (MLPHARE DM)
Refinement software	CNX (2000.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.510
Number of reflections	22893
Completeness [%]	87.2	79.4
Redundancy	2.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN FORMULATED AT 10 MG/ML WAS CRYSTALLIZED WITH 100 MM TRIS PH 8.0, 25% PEG 3350, 80 MM MGCL2, 8% METHANOL, 10% GLYCEROL AND 5 MM DTT.