2JFV

Crystal structure of Enterococcus faecium glutamate racemase in complex with citrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-11
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	85.160, 85.160, 92.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.000 - 1.800
Rwork	0.199
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jfu
RMSD bond length	0.005
RMSD bond angle	1.301
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNX (2000.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.200
Number of reflections	34849
<I/σ(I)>	10.1	3.7
Completeness [%]	95.5	95.5
Redundancy	4.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1