2JFQ
Crystal structure of Staphylococcus aureus glutamate racemase in complex with D- Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.430, 88.870, 96.560 |
| Unit cell angles | 90.00, 109.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 2.150 |
| Rwork | 0.202 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jfp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.191 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNX (2000.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.220 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.100 | 0.340 |
| Number of reflections | 41628 | |
| <I/σ(I)> | 6.7 | 2.2 |
| Completeness [%] | 98.2 | 90.7 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | PROTEIN FORMULATED AT 10 MG/ML WITH 200 MM AMMONIUM ACETATE PH 7.4, 5 MM D-L GLUTAMATE, 1 MM TCEP AND CRYSTALLISED WITH 0.17 MM AMMONIUM SULPHATE AND 25% PEG 8000 |
