2JFB
3D Structure of Lumazine Synthase from Candida albicans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-02 |
Detector | MARRESEACH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 128.648, 128.648, 284.965 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.213 |
Rwork | 0.210 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ejb |
RMSD bond length | 0.006 |
RMSD bond angle | 0.886 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 | 0.490 |
Number of reflections | 94547 | |
<I/σ(I)> | 9 | 1.95 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.00 |