2JF7
Structure of Strictosidine Glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 157.630, 157.630, 103.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.480 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e1e |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.090 | 0.270 |
| Number of reflections | 46616 | |
| <I/σ(I)> | 14.6 | 4.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.3 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.35 M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE PH 4.6 PEG4000 |
