2JF6
Structure of inactive mutant of Strictosidine Glucosidase in complex with strictosidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 159.000, 159.000, 102.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.820 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jf7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.624 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.060 | 0.110 |
| Number of reflections | 32951 | |
| <I/σ(I)> | 20.2 | 7.6 |
| Completeness [%] | 98.4 | 99.1 |
| Redundancy | 28.2 | 28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.35 M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE PH 4.6 11% PEG4000 |
