2JEX

Transcription activator structure reveals redox control of a replication initiation reaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	120
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	60.549, 60.549, 89.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.620 - 2.350
R-factor	0.211
Rwork	0.210
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jeu
RMSD bond length	0.010
RMSD bond angle	1.073
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.070	0.320
Number of reflections	14352
<I/σ(I)>	10.7	2.5
Completeness [%]	93.3	90.2
Redundancy	1.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5		PROTEIN WAS CRYSTALLISED FROM 0.1 M TRIS-HCL PH 8.5, 0.3 M NACL, 2MM DTT AND 18-22% TERTIARY BUTANOL, USING THE HANGING DROP METHOD OF VAPOUR DIFFUSION.