2JEO
Crystal structure of human uridine-cytidine kinase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 67.971, 67.971, 236.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.450 - 2.500 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rxj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.191 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.460 |
| Number of reflections | 12014 | |
| <I/σ(I)> | 25.4 | 7.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 18.6 | 17.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.8-1.0 M SUCCINIC ACID PH 6.7-7.3 |
