2JEN
Family 12 xyloglucanase from Bacillus licheniformis in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-04 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 114.629, 114.629, 110.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.540 - 1.400 |
| R-factor | 0.116 |
| Rwork | 0.115 |
| R-free | 0.14100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jem |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.731 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 54792 | |
| <I/σ(I)> | 27.3 | 3.6 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 8.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.6M AMMONIUM SULPHATE, 10% DIOXANE, 0.1M MES, PH 6.5 |
