2JEK
Crystal structure of the conserved hypothetical protein Rv1873 from Mycobacterium tuberculosis at 1.38 A
Replaces: 2D2YExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.440, 31.627, 53.187 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.380 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.166 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.030 | 0.400 |
| Number of reflections | 21820 | |
| <I/σ(I)> | 15.5 | 2 |
| Completeness [%] | 94.2 | 85.8 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | AMMONIUM SULPHATE, MES BUFFER, GLYCEROL, ETHYLEN GLYCOL, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
