2JE6
Structure of a 9-subunit archaeal exosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 135.490, 135.490, 135.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.180 - 1.600 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.100 | 0.680 |
| Number of reflections | 106599 | |
| <I/σ(I)> | 11.7 | 2.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 40% PEG 400, 50 MM TRIS-HCL PH 8.0 |
