2JE2
Cytochrome P460 from Nitrosomonas europaea - probable nonphysiological oxidized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-05 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.256, 53.256, 127.033 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.360 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INITIAL MODEL FROM SULPHUR SAD STRUCTURE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.641 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.040 | 0.100 |
| Number of reflections | 17631 | |
| <I/σ(I)> | 17.7 | 2.8 |
| Completeness [%] | 80.9 | 13.5 |
| Redundancy | 2.9 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | pH 5.20 |
