2JCQ
The hyaluronan binding domain of murine CD44 in a Type A complex with an HA 8-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.794, 82.051, 32.278 |
| Unit cell angles | 90.00, 117.89, 90.00 |
Refinement procedure
| Resolution | 41.030 - 1.250 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.15600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uuh |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.774 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.420 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.070 | 0.420 |
| Number of reflections | 36225 | |
| <I/σ(I)> | 6.75 | 1.77 |
| Completeness [%] | 92.7 | 56.2 |
| Redundancy | 5.44 | 2.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | CO-CRYSTALS OF THE CD44/HA8 COMPLEX WERE PREPARED AFTER ADDITION OF HA8 (2MM FINAL CONCENTRATION) TO THE 0.5MM PROTEIN SOLUTION FOLLOWED BY MIXING 1:1 WITH WELL SOLUTIONS CONTAINING 25% (W/V) PEG 3350 AND 100MM NACL IN 100MM HEPES BUFFERED AT EITHER PH 7.0 OR PH 8.0 |
