2JBX
Crystal Structure of the myxoma virus anti-apoptotic protein M11L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-07 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.876, 39.398, 49.222 |
| Unit cell angles | 71.79, 90.09, 81.66 |
Refinement procedure
| Resolution | 20.000 - 2.730 |
| R-factor | 0.218 |
| Rwork | 0.214 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | M11L BAK COMPLEX |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.870 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.830 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.110 | 0.350 |
| Number of reflections | 7483 | |
| <I/σ(I)> | 9 | 3 |
| Completeness [%] | 97.3 | 91.6 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 1.8 M AMMONIUM SULFATE, 10 MM KSCN, 100 MM HEPES PH 7.2 |
