2JBH
Human phosphoribosyl transferase domain containing 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 139.232, 139.232, 52.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.440 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hmp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.398 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.100 | 0.460 |
| Number of reflections | 63918 | |
| <I/σ(I)> | 19.4 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 100 MM SODIUM CACODYLATE PH 6.1, 200 MM CALCIUM ACETATE, 34% PEG 300 |
