2JB7
PAE2307 with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 120.133, 120.133, 156.251 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.350 - 1.650 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wvq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.739 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 68521 | |
| <I/σ(I)> | 27.9 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.7 | PAE2307(6MG/ML) WITH 0.5MM AMP IN 21% PEG4000, 200MM SODIUM ACETATE TRIHYDRATE AND 100 MM TRIS HCL; PH 8.7 |
